TY - JOUR
T1 - Stereology techniques in radiation biology
AU - Kubínová, Lucie
AU - Mao, Xiao Wen
AU - Janáček, Jiří
AU - Archambeau, John O.
N1 - Lucie Kubínová, XiaoWen Mao, Jiří Janáček, and John O. Archambeau (2003) Stereology Techniques in Radiation Biology. Radiation Research: Vol. 160, No. 1, pp. 110-119. https://doi.org/10.1667/R3016
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Clinicians involved in conventional radiation therapy are very concerned about the dose-response relationships of normal tissues. Before proceeding to new clinical protocols, radiation biologists involved with conformal proton therapy believe it is necessary to quantify the dose response and tolerance of the organs and tissues that will be irradiated. An important focus is on the vasculature. This presentation reviews the methodology and format of using confocal microscopy and stereological methods to quantify tissue parameters, cell number, tissue volume and surface area, and vessel length using the microvasculature as a model tissue. Stereological methods and their concepts are illustrated using an ongoing study of the dose response of the microvessels in proton-irradiated hemibrain. Methods for estimating the volume of the brain and the brain cortex, the total number of endothelial cells in cortical microvessels, the length of cortical microvessels, and the total surface area of cortical microvessel walls are presented step by step in a way understandable for readers with little mathematical background. It is shown that stereological techniques, based on a sound theoretical basis, are powerful and reliable and have been used successfully.
AB - Clinicians involved in conventional radiation therapy are very concerned about the dose-response relationships of normal tissues. Before proceeding to new clinical protocols, radiation biologists involved with conformal proton therapy believe it is necessary to quantify the dose response and tolerance of the organs and tissues that will be irradiated. An important focus is on the vasculature. This presentation reviews the methodology and format of using confocal microscopy and stereological methods to quantify tissue parameters, cell number, tissue volume and surface area, and vessel length using the microvasculature as a model tissue. Stereological methods and their concepts are illustrated using an ongoing study of the dose response of the microvessels in proton-irradiated hemibrain. Methods for estimating the volume of the brain and the brain cortex, the total number of endothelial cells in cortical microvessels, the length of cortical microvessels, and the total surface area of cortical microvessel walls are presented step by step in a way understandable for readers with little mathematical background. It is shown that stereological techniques, based on a sound theoretical basis, are powerful and reliable and have been used successfully.
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U2 - 10.1667/R3016
DO - 10.1667/R3016
M3 - Review article
C2 - 12816530
SN - 0033-7587
VL - 160
SP - 110
EP - 119
JO - Radiation Research
JF - Radiation Research
IS - 1
ER -