TY - JOUR
T1 - Spinophilin participates in information transfer at immunological synapses
AU - Bloom, Ona
AU - Unternaehrer, Julia J.
AU - Jiang, Aimin
AU - Shin, Jeong Sook
AU - Delamarre, Lélia
AU - Allen, Patrick
AU - Mellman, Ira
N1 - We found that spinophilin mRNA is expressed by a variety of immune cells in mice, including DCs, macrophages, B cells, and T cells, by RT-PCR (unpublished data). At the protein level, we could detect the same 135-kD band in lysates of brain-, spleen-, and bone marrow-derived DCs by Western blotting ().
PY - 2008/4/21
Y1 - 2008/4/21
N2 - The adaptive immune response is initiated by the presentation of peptides bound to major histocompatibility complex molecules on dendritic cells (DCs) to antigen-specific T lymphocytes at a junction termed the immunological synapse. Although much attention has been paid to cytoplasmic events on the T cell side of the synapse, little is known concerning events on the DC side. We have sought signal transduction components of the neuronal synapse that were also expressed by DCs. One such protein is spinophilin, a scaffolding protein of neuronal dendritic spines that regulates synaptic transmission. In inactive, immature DCs, spinophilin is located throughout the cytoplasm but redistributes to the plasma membrane upon stimulus-induced maturation. In DCs interacting with T cells, spinophilin is polarized dynamically to contact sites in an antigen-dependent manner. It is also required for optimal T cell activation because DCs derived from mice lacking spinophilin exhibit defects in antigen presentation both in vitro and in vivo. Thus, spinophilin may play analogous roles in information transfer at both neuronal and immunological synapses.
AB - The adaptive immune response is initiated by the presentation of peptides bound to major histocompatibility complex molecules on dendritic cells (DCs) to antigen-specific T lymphocytes at a junction termed the immunological synapse. Although much attention has been paid to cytoplasmic events on the T cell side of the synapse, little is known concerning events on the DC side. We have sought signal transduction components of the neuronal synapse that were also expressed by DCs. One such protein is spinophilin, a scaffolding protein of neuronal dendritic spines that regulates synaptic transmission. In inactive, immature DCs, spinophilin is located throughout the cytoplasm but redistributes to the plasma membrane upon stimulus-induced maturation. In DCs interacting with T cells, spinophilin is polarized dynamically to contact sites in an antigen-dependent manner. It is also required for optimal T cell activation because DCs derived from mice lacking spinophilin exhibit defects in antigen presentation both in vitro and in vivo. Thus, spinophilin may play analogous roles in information transfer at both neuronal and immunological synapses.
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U2 - 10.1083/jcb.200711149
DO - 10.1083/jcb.200711149
M3 - Article
C2 - 18411312
SN - 0021-9525
VL - 181
SP - 203
EP - 211
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 2
ER -