TY - JOUR
T1 - Salmonella phage PSP3, another member of the P2-like phage group
AU - Bullas, Leonard R.
AU - Mostaghimi, Ali Reza
AU - Arensdorf, Joseph J.
AU - Rajadas, Phillip T.
AU - Zuccarelli, Anthony J.
N1 - Funding Information:
We gratefully acknowledge the technical assistance of Erica Cour-tright, who was supported by a grant from the California Museum of Science and Industry, Rhonda Hansen, and Dean Lee. We thank Drs. Egan and Dodd of The University of Adelaide, Australia, for supplying the sequence around cos for phage 186. The research was supported in part by Public Health Service Research Grant RR05352 from the National Institutes of Health to Loma Linda University School of Medicine.
PY - 1991/12
Y1 - 1991/12
N2 - Freshly isolated DNA of phage PSP3, whose morphology closely resembles that of phage P2, contained both circular and linear molecules about 31 kb in length. Linear PSP3 DNA molecules possess single-stranded cohesive termini (cos). Sequencing of the fragment anticipated to contain cos revealed a 19-base sequence identical to cos of phage 186. Of the 107 by to the right of cos, 94 were identical in 186 DNA (88% similarity), and of the 370 by to the left, 229 were identical (62% similarity). Cos flanking sequences in both P2 and P4 were also highly conserved in PSP3. A number of restriction sites were at similar locations on the two phage DNAs. The parasitic phage P4 propagated on PSP3 lysogens. PSP3 integrates into the Escherichia coli chromosome at 27 min.
AB - Freshly isolated DNA of phage PSP3, whose morphology closely resembles that of phage P2, contained both circular and linear molecules about 31 kb in length. Linear PSP3 DNA molecules possess single-stranded cohesive termini (cos). Sequencing of the fragment anticipated to contain cos revealed a 19-base sequence identical to cos of phage 186. Of the 107 by to the right of cos, 94 were identical in 186 DNA (88% similarity), and of the 370 by to the left, 229 were identical (62% similarity). Cos flanking sequences in both P2 and P4 were also highly conserved in PSP3. A number of restriction sites were at similar locations on the two phage DNAs. The parasitic phage P4 propagated on PSP3 lysogens. PSP3 integrates into the Escherichia coli chromosome at 27 min.
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U2 - 10.1016/0042-6822(91)90573-T
DO - 10.1016/0042-6822(91)90573-T
M3 - Article
C2 - 1962462
SN - 0042-6822
VL - 185
SP - 918
EP - 921
JO - Virology
JF - Virology
IS - 2
ER -