Reprogramming of human cord blood CD34+ cells into induced MSCs

Amanda Neises; Ruijun Jeanna Su; Xiao Bing Zhang

doi:10.1007/978-94-017-7312-6_7

Reprogramming of human cord blood CD34⁺ cells into induced MSCs

Amanda Neises, Ruijun Jeanna Su, Xiao Bing Zhang

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Blood cells are the most accessible cells for reprogramming. Mesenchymal stem cells (MSCs) have multiple applications in regenerative medicine. We reported that induced MSCs (iMSCs) can be efficiently generated with OCT4 under defined conditions and iMSCs can be immortalized by overexpression of OCT4. Here, we detail the protocol for generating integration-free iMSCs from cord blood CD34⁺ cells using an episomal vector and describe the approach to immortalizing iMSCs.

Original language	English
Title of host publication	Hematopoietic Differentiation of Human Pluripotent Stem Cells
Publisher	Springer Netherlands
Pages	91-101
Number of pages	11
ISBN (Electronic)	9789401773126
ISBN (Print)	9789401773119
DOIs	https://doi.org/10.1007/978-94-017-7312-6_7
State	Published - Aug 25 2015

ASJC Scopus Subject Areas

General Medicine
General Immunology and Microbiology
General Biochemistry,Genetics and Molecular Biology

Keywords

CD34 cells
Episomal vector
Hematopoietic stem/progenitor cells
Mesenchymal stem cells
OCT4
Reprogramming

Access to Document

10.1007/978-94-017-7312-6_7License: Unspecified

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Cite this

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