Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis

Melissa Gulmezian; Haitao Zhang; George T. Javor; Catherine F. Clarke

doi:10.1128/JB.00668-06

Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis

Melissa Gulmezian, Haitao Zhang, George T. Javor, Catherine F. Clarke

Biochemistry and Microbiology

Research output: Contribution to journal › Article › peer-review

Abstract

IS16 is a thiol-sensitive, Q-deficient mutant strain of Escherichia coli. Here, we show that IS16 harbors a mutation in the ubiG gene encoding a methyltransferase required for two O-methylation steps of Q biosynthesis. Complementation of IS16 with either ubiG or ubiX_K-12 reverses this phenotype, suggesting that UbiX may interact with UbiG.

Original language	English
Pages (from-to)	6435-6439
Number of pages	5
Journal	Journal of Bacteriology
Volume	188
Issue number	17
DOIs	https://doi.org/10.1128/JB.00668-06
State	Published - Sep 2006

ASJC Scopus Subject Areas

Microbiology
Molecular Biology

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Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis

Abstract

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