Generation of iPS cells from human peripheral blood mononuclear cells using episomal vectors

Ruijun Jeanna Su, Amanda Neises, Xiao Bing Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Peripheral blood is the easy-to-access, minimally invasive, and the most abundant cell source to use for cell reprogramming. The episomal vector is among the best approaches for generating integration-free induced pluripotent stem (iPS) cells due to its simplicity and affordability. Here we describe the detailed protocol for the efficient generation of integration-free iPS cells from peripheral blood mononuclear cells. With this optimized protocol, one can readily generate hundreds of iPS cell colonies from 1 ml of peripheral blood.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages57-69
Number of pages13
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1357
ISSN (Print)1064-3745

ASJC Scopus Subject Areas

  • Molecular Biology
  • Genetics

Keywords

  • Episomal vectors
  • Hematopoietic cells
  • Human peripheral blood
  • Induced pluripotent stem cells
  • Integration-free
  • Mononuclear cells
  • Reprogramming

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