Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Wei Wen; Jian-Ping Zhang; Wanqiu Chen; Cameron Arakaki; Xiaolan Li; David J. Baylink; Gary D. Botimer; Jing Xu; Weiping Yuan; Tao Cheng; Xiao-Bing Zhang

doi:10.3791/55091

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Wei Wen, Jian-Ping Zhang, Wanqiu Chen, Cameron Arakaki, Xiaolan Li, David J. Baylink, Gary D. Botimer, Jing Xu, Weiping Yuan, Tao Cheng, Xiao-Bing Zhang

Research output: Non-textual form › Digital or Visual Products

Original language	American English
Media of output	Online
DOIs	https://doi.org/10.3791/55091
State	Published - Jan 1 2017

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Molecular Biology
Biology

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@misc{3ed53ba37c964c0e900c634cf007a7d1,

title = "Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors",

author = "Wei Wen and Jian-Ping Zhang and Wanqiu Chen and Cameron Arakaki and Xiaolan Li and Baylink, {David J.} and Botimer, {Gary D.} and Jing Xu and Weiping Yuan and Tao Cheng and Xiao-Bing Zhang",

note = "This protocol describes a detailed method for efficient generation of integration-free iPSCs from human adult peripheral blood cells. With the use of four oriP/EBNA-based episomal vectors to express the reprogramming factors, KLF4, MYC, BCL-XL, or OCT4 and SOX2, thousands of iPSC colonies can be obtained from 1 mL of peripheral blood.",

year = "2017",

month = jan,

day = "1",

doi = "10.3791/55091",

language = "American English",

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TY - ADVS

T1 - Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

AU - Wen, Wei

AU - Zhang, Jian-Ping

AU - Chen, Wanqiu

AU - Arakaki, Cameron

AU - Li, Xiaolan

AU - Baylink, David J.

AU - Botimer, Gary D.

AU - Xu, Jing

AU - Yuan, Weiping

AU - Cheng, Tao

AU - Zhang, Xiao-Bing

N1 - This protocol describes a detailed method for efficient generation of integration-free iPSCs from human adult peripheral blood cells. With the use of four oriP/EBNA-based episomal vectors to express the reprogramming factors, KLF4, MYC, BCL-XL, or OCT4 and SOX2, thousands of iPSC colonies can be obtained from 1 mL of peripheral blood.

PY - 2017/1/1

Y1 - 2017/1/1

UR - https://www.jove.com/t/55091/generation-integration-free-induced-pluripotent-stem-cells-from-human

U2 - 10.3791/55091

DO - 10.3791/55091

M3 - Digital or Visual Products

ER -

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

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