TY - JOUR
T1 - Enzymatic basis of thiol-stimulated secretion of porphyrins by Escherichia coli
AU - Javor, G. T.
AU - Febre, E. F.
N1 - By G T Javor and E F Febre 1-Thioglycerol (TG) stimulates the synthesis of porphyrin in aerobically growing Escherichia coli. Here the levels of delta-aminolevulinate biosynthetic enzymes in untreated and TG-treated E. coli THU and PUC2 (a mutant of THU which overproduces porphyrins in the presence of thiols) cells were determined.
PY - 1992
Y1 - 1992
N2 - 1-Thioglycerol (TG) stimulates the synthesis of porphyrin in aerobically growing Escherichia coli. Here the levels of delta-aminolevulinate biosynthetic enzymes in untreated and TG-treated E. coli THU and PUC2 (a mutant of THU which overproduces porphyrins in the presence of thiols) cells were determined. TG treatment elevated the activity of glutamyl-tRNA reductase in both strains. The increased activity was not caused by activation of preexisting enzymes by thiols or by oxidizing agents but was dependent on new protein synthesis.
AB - 1-Thioglycerol (TG) stimulates the synthesis of porphyrin in aerobically growing Escherichia coli. Here the levels of delta-aminolevulinate biosynthetic enzymes in untreated and TG-treated E. coli THU and PUC2 (a mutant of THU which overproduces porphyrins in the presence of thiols) cells were determined. TG treatment elevated the activity of glutamyl-tRNA reductase in both strains. The increased activity was not caused by activation of preexisting enzymes by thiols or by oxidizing agents but was dependent on new protein synthesis.
UR - http://www.scopus.com/inward/record.url?scp=0026526699&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026526699&partnerID=8YFLogxK
U2 - 10.1128/jb.174.3.1072-1075.1992
DO - 10.1128/jb.174.3.1072-1075.1992
M3 - Comment/debate
C2 - 1732201
SN - 0021-9193
VL - 174
SP - 1072
EP - 1075
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 3
ER -