Effects of inactivating Sp6 and Sp8 genes on dorsal-ventral limb patterning.

Haro Endika; Delgado Irene; Junco Marisa; Yamada Yoshihiko; Mansouri Ahmed; C. Oberg Kerby; A. Ros Marian; Kerby C. Oberg

doi:10.1371/journal.pgen.1004468.g006

Effects of inactivating Sp6 and Sp8 genes on dorsal-ventral limb patterning.

Haro Endika, Delgado Irene, Junco Marisa, Yamada Yoshihiko, Mansouri Ahmed, C. Oberg Kerby, A. Ros Marian, Kerby C. Oberg

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Abstract

 ISH to transverse sections through the level of the forelimbs at the stage indicated at the top and with the probe indicated on the left. Genotypes are also marked at the top. Note that, contrary to controls (A, E),    Wnt7a   is not restricted to the dorsal ectoderm in double mutant embryos (B, F). Accordingly,    En1   expression is undetectable in the ventral limb ectoderm of mutant embryos (D, H) compared to controls (C, G). The arrowheads and arrows mark the distal limit of    Wnt7a   and    En1   expression, respectively.
 
 

Original language	American English
Media of output	Online
DOIs	https://doi.org/10.1371/journal.pgen.1004468.g006
State	Published - Aug 28 2014

Keywords

sp 6
Sp 8 activity
Sp 8 Transcription Factors Control AER Formation
transcription factors Sp 6
Wnt 7a expression
mutants show defects
Sp 8
role Sp 6
apical ectodermal ridge
Limb development
Sp 8 mutants exhibit
limb ectoderm
dorsal digit tips

Disciplines

Molecular Biology
Biology

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title = "Effects of inactivating Sp6 and Sp8 genes on dorsal-ventral limb patterning.",

abstract = " ISH to transverse sections through the level of the forelimbs at the stage indicated at the top and with the probe indicated on the left. Genotypes are also marked at the top. Note that, contrary to controls (A, E), Wnt7a is not restricted to the dorsal ectoderm in double mutant embryos (B, F). Accordingly, En1 expression is undetectable in the ventral limb ectoderm of mutant embryos (D, H) compared to controls (C, G). The arrowheads and arrows mark the distal limit of Wnt7a and En1 expression, respectively. ",

keywords = "sp 6, Sp 8 activity, Sp 8 Transcription Factors Control AER Formation, transcription factors Sp 6, Wnt 7a expression, mutants show defects, Sp 8, role Sp 6, apical ectodermal ridge, Limb development, Sp 8 mutants exhibit, limb ectoderm, dorsal digit tips",

author = "Haro Endika and Delgado Irene and Junco Marisa and Yamada Yoshihiko and Mansouri Ahmed and Kerby, {C. Oberg} and Marian, {A. Ros} and Oberg, {Kerby C.}",

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AU - Irene, Delgado

AU - Marisa, Junco

AU - Yoshihiko, Yamada

AU - Ahmed, Mansouri

AU - Kerby, C. Oberg

AU - Marian, A. Ros

AU - Oberg, Kerby C.

N1 - ISH to transverse sections through the level of the forelimbs at the stage indicated at the top and with the probe indicated on the left. Genotypes are also marked at the top. Note that, contrary to controls (A, E), Wnt7a is not restricted to the dorsal ectoderm in double mutant embryos (B, F).

PY - 2014/8/28

Y1 - 2014/8/28

N2 - ISH to transverse sections through the level of the forelimbs at the stage indicated at the top and with the probe indicated on the left. Genotypes are also marked at the top. Note that, contrary to controls (A, E), Wnt7a is not restricted to the dorsal ectoderm in double mutant embryos (B, F). Accordingly, En1 expression is undetectable in the ventral limb ectoderm of mutant embryos (D, H) compared to controls (C, G). The arrowheads and arrows mark the distal limit of Wnt7a and En1 expression, respectively.

AB - ISH to transverse sections through the level of the forelimbs at the stage indicated at the top and with the probe indicated on the left. Genotypes are also marked at the top. Note that, contrary to controls (A, E), Wnt7a is not restricted to the dorsal ectoderm in double mutant embryos (B, F). Accordingly, En1 expression is undetectable in the ventral limb ectoderm of mutant embryos (D, H) compared to controls (C, G). The arrowheads and arrows mark the distal limit of Wnt7a and En1 expression, respectively.

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KW - Sp 8 activity

KW - Sp 8 Transcription Factors Control AER Formation

KW - transcription factors Sp 6

KW - Wnt 7a expression

KW - mutants show defects

KW - Sp 8

KW - role Sp 6

KW - apical ectodermal ridge

KW - Limb development

KW - Sp 8 mutants exhibit

KW - limb ectoderm

KW - dorsal digit tips

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M3 - Digital or Visual Products

ER -

Effects of inactivating Sp6 and Sp8 genes on dorsal-ventral limb patterning.

Abstract

Keywords

Disciplines

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