TY - GEN
T1 - Effect of recombinant osteopontin on cerebral vasospasm after subarachnoid hemorrhage in rats
AU - Suzuki, Hidenori
AU - Hasegawa, Yu
AU - Kanamaru, Kenji
AU - Zhang, John H.
N1 - Funding Information:
This study was partially supported by grants (NS053407) from the National Institutes of Health to J.H.Z.
PY - 2011
Y1 - 2011
N2 - Background: Osteopontin (OPN), a pleiotropic extracellular matrix glycoprotein, has been reported to have neuroprotective effects against early brain injury after subarachnoid hemorrhage (SAH). The aim of this study is to examine if osteopontin prevents cerebral vasospasm after SAH in rats. Method: The endovascular perforation model of SAH was produced, and 62 rats were randomly assigned to sham + vehicle, SAH + vehicle, and SAH+ r-OPN (0.1 μg) groups. Cerebral vasospasm was evaluated by India ink angiography at 24 and 72 h after SAH, as well as neurobehavioral tests. Findings: Significant vasospasm and neurological impairments occurred over the observed period after SAH. r-OPN significantly prevented vasospasm in the left middle cerebral artery at 24 h and improved neurological impairments at 48 h after SAH. In other time points studied, r-OPN had a tendency toward improving both vasospasm and neurological scores, but the difference was not significant. Conclusions: This study shows that r-OPN has anti-vasospastic effects against cerebral vasospasm after SAH.
AB - Background: Osteopontin (OPN), a pleiotropic extracellular matrix glycoprotein, has been reported to have neuroprotective effects against early brain injury after subarachnoid hemorrhage (SAH). The aim of this study is to examine if osteopontin prevents cerebral vasospasm after SAH in rats. Method: The endovascular perforation model of SAH was produced, and 62 rats were randomly assigned to sham + vehicle, SAH + vehicle, and SAH+ r-OPN (0.1 μg) groups. Cerebral vasospasm was evaluated by India ink angiography at 24 and 72 h after SAH, as well as neurobehavioral tests. Findings: Significant vasospasm and neurological impairments occurred over the observed period after SAH. r-OPN significantly prevented vasospasm in the left middle cerebral artery at 24 h and improved neurological impairments at 48 h after SAH. In other time points studied, r-OPN had a tendency toward improving both vasospasm and neurological scores, but the difference was not significant. Conclusions: This study shows that r-OPN has anti-vasospastic effects against cerebral vasospasm after SAH.
KW - Casting method
KW - Cerebral vasospasm
KW - Osteopontin
KW - Subarachnoid hemorrhage
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U2 - 10.1007/978-3-7091-0356-2_6
DO - 10.1007/978-3-7091-0356-2_6
M3 - Conference contribution
C2 - 21125441
SN - 9783709103555
T3 - Acta Neurochirurgica, Supplementum
SP - 29
EP - 32
BT - Early Brain Injury or Cerebral Vasospasm
PB - Springer-Verlag Wien
ER -