Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing

Juan Juan Zhao; Xin Yu Sun; Sai Ning Tian; Zong Ze Zhao; Meng Di Yin; Mei Zhao; Feng Zhang; Si Ang Li; Zhi Xue Yang; Wei Wen; Tao Cheng; An Gong; Jian Ping Zhang; Xiao Bing Zhang

doi:10.1186/s12864-024-10050-6

Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing

Juan Juan Zhao, Xin Yu Sun, Sai Ning Tian, Zong Ze Zhao, Meng Di Yin, Mei Zhao, Feng Zhang, Si Ang Li, Zhi Xue Yang, Wei Wen, Tao Cheng, An Gong, Jian Ping Zhang, Xiao Bing Zhang

Research output: Contribution to journal › Article › peer-review

Original language	English
Article number	189
Journal	BMC Genomics
Volume	25
Issue number	1
DOIs	https://doi.org/10.1186/s12864-024-10050-6
State	Published - Dec 2024
Externally published	Yes

ASJC Scopus Subject Areas

Biotechnology
Genetics

Keywords

CRISPR-Cas9
DNA integration
Gene therapy
Long-range PCR
Nanopore sequencing

Access to Document

10.1186/s12864-024-10050-6

OpenUrl availability

Full text

Cite this

Zhao, J. J., Sun, X. Y., Tian, S. N., Zhao, Z. Z., Yin, M. D., Zhao, M., Zhang, F., Li, S. A., Yang, Z. X., Wen, W., Cheng, T., Gong, A., Zhang, J. P., & Zhang, X. B. (2024). Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing. BMC Genomics, 25(1), Article 189. https://doi.org/10.1186/s12864-024-10050-6

@article{111d750f7f884de58e766e53e087199e,

title = "Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing",

keywords = "CRISPR-Cas9, DNA integration, Gene therapy, Long-range PCR, Nanopore sequencing",

author = "Zhao, {Juan Juan} and Sun, {Xin Yu} and Tian, {Sai Ning} and Zhao, {Zong Ze} and Yin, {Meng Di} and Mei Zhao and Feng Zhang and Li, {Si Ang} and Yang, {Zhi Xue} and Wei Wen and Tao Cheng and An Gong and Zhang, {Jian Ping} and Zhang, {Xiao Bing}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",

year = "2024",

month = dec,

doi = "10.1186/s12864-024-10050-6",

language = "English",

volume = "25",

journal = "BMC Genomics",

issn = "1471-2164",

number = "1",

}

TY - JOUR

T1 - Decoding the complexity of on-target integration

T2 - characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing

AU - Zhao, Juan Juan

AU - Sun, Xin Yu

AU - Tian, Sai Ning

AU - Zhao, Zong Ze

AU - Yin, Meng Di

AU - Zhao, Mei

AU - Zhang, Feng

AU - Li, Si Ang

AU - Yang, Zhi Xue

AU - Wen, Wei

AU - Cheng, Tao

AU - Gong, An

AU - Zhang, Jian Ping

AU - Zhang, Xiao Bing

PY - 2024/12

Y1 - 2024/12

KW - CRISPR-Cas9

KW - DNA integration

KW - Gene therapy

KW - Long-range PCR

KW - Nanopore sequencing

UR - http://www.scopus.com/inward/record.url?scp=85185403958&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85185403958&partnerID=8YFLogxK

U2 - 10.1186/s12864-024-10050-6

DO - 10.1186/s12864-024-10050-6

M3 - Article

C2 - 38368357

AN - SCOPUS:85185403958

SN - 1471-2164

VL - 25

JO - BMC Genomics

JF - BMC Genomics

IS - 1

M1 - 189

ER -

Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing

ASJC Scopus Subject Areas

Keywords

Access to Document

OpenUrl availability

Other files and links

Cite this