TY - JOUR
T1 - Chlorisondamine, a sympathetic ganglionic blocker, moderates the effects of whole-body irradiation (WBI) on early host defense to a live bacterial challenge
AU - Pecaut, Michael J.
AU - Mehrotra, Shalini
AU - Luo-Owen, Xian
AU - Bayeta, Erben J.M.
AU - Bellinger, Denise L.
AU - Gridley, Daila S.
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - There is a growing consensus that long-term deficits in the brain are due to dynamic interactions between multiple neural and immune cell types. Specifically, radiation induces an inflammatory response, including changes in neuromodulatory pro- and anti-inflammatory cytokine secretion. The purpose of this study was to establish that there is sympathetic involvement in radiation-induced decrements early in in vivo immune function host defense. Female, 8-9 week-old C57BL/6J mice were exposed to whole-body irradiation (WBI). There were 8 groups with radiation (0 vs. 3. Gy protons), immune challenge (. Escherichia coli) and exposure to the sympathetic ganglionic blocker, chlorisondamine (1. mg/kg weight, i.p.), as independent variables. Ten days post-irradiation, mice were inoculated with E. coli intraperitoneally and sacrificed 90-120. min later. The data suggest that radiation-induced changes in immune function may in part be mediated by the sympathetic nervous system. Briefly, we found that radiation augments the bacteria-induced inflammatory cytokine response, particularly those cytokines involved in innate immunity. However, this augmentation can be reduced by the ganglionic blockade.
AB - There is a growing consensus that long-term deficits in the brain are due to dynamic interactions between multiple neural and immune cell types. Specifically, radiation induces an inflammatory response, including changes in neuromodulatory pro- and anti-inflammatory cytokine secretion. The purpose of this study was to establish that there is sympathetic involvement in radiation-induced decrements early in in vivo immune function host defense. Female, 8-9 week-old C57BL/6J mice were exposed to whole-body irradiation (WBI). There were 8 groups with radiation (0 vs. 3. Gy protons), immune challenge (. Escherichia coli) and exposure to the sympathetic ganglionic blocker, chlorisondamine (1. mg/kg weight, i.p.), as independent variables. Ten days post-irradiation, mice were inoculated with E. coli intraperitoneally and sacrificed 90-120. min later. The data suggest that radiation-induced changes in immune function may in part be mediated by the sympathetic nervous system. Briefly, we found that radiation augments the bacteria-induced inflammatory cytokine response, particularly those cytokines involved in innate immunity. However, this augmentation can be reduced by the ganglionic blockade.
KW - Chlorisondamine
KW - Cytokines
KW - Protons
KW - Sympathetic nervous system
KW - Whole-body irradiation
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U2 - 10.1016/j.imlet.2015.07.008
DO - 10.1016/j.imlet.2015.07.008
M3 - Article
C2 - 26235133
SN - 0165-2478
VL - 167
SP - 103
EP - 115
JO - Immunology Letters
JF - Immunology Letters
IS - 2
ER -